Core Facility — Proteomic Mass Spectrometry
Mass Spectrometers
The Core Facility - Proteomic Mass Spectrometry at Weinberg Campus are equipped with state-of-the-art chromatography systems and mass spectrometers
This is our principle peptide measuring instrument.
label and label-free quantification
10000 proteins in 100 min with BoxCar
Multiplexing ≥ 20 precursors per scan
Mass range m/z 40–8000
Complex samples in a single analysis
FAIMS
Scan Rate up to 40 Hz
Dynamic range: >5000
Mass resolving power 480,000 FWHM @ 200 m/z
Label-free quantification and identification of complex protein mixtures such as cell lysates and pull downs.
High Definition Mass Spectrometer
High-sensitivity StepWave ion transfer
High-Definition MSE acquisition
Complex samples in a single analysis
Triwave ion mobility
Linear dynamic range: 105
Mass resolving power >40,000 FWHM
A triple Quadropole mass spectrometer designed specifically for high sensitivity targeted proteomics such as MRMs.
This Mass Spectrometer is shared with Department of Enzymology (Mike Schutkowski's Lab)
bench top
tandem quadrupole MS
ultra high performance LC-MS/MS
T-Wave collision cell
high-speed MRM
This is a MALDI instrument designed to measure small proteins, simple peptide mixtures andsmall molecules.
mass resolving up to 40,000
High acquisition speed in MS & MS/MS
FlashDetector & 5 GS/s 10-bit digitizer
IR-laser based self-cleaning ion source
full MS/MS can be acquired with up to 1000 Hz lase
repetition rate
This quadrupole ICP-MS is shared with and oparated by Dr. Martin Herzberg (Dietrich H. Nies' Lab).